Subba Rao, Chaganti ; Shetty Prakasham, Reddy ; Bhaskar Rao, Adari ; Yadav, Jhillu S. (2007) Production of L (+) lactic acid by Lactobacillus delbrueckii immobilized in functionalized alginate matrices World Journal of Microbiology and Biotechnology, 24 (8). pp. 1411-1415. ISSN 0959-3993
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Official URL: http://www.springerlink.com/content/u3217601155686...
Related URL: http://dx.doi.org/10.1007/s11274-007-9623-0
Abstract
The role of functionalized alginate gels as immobilized matrices in production of L (+) lactic acid by Lactobacillus delbrueckii was studied. L. delbrueckii cells immobilized in functionalized alginate beads showed enhanced bead stability and selectivity towards production of optically pure L (+) lactic acid in higher yields (1.74Yp/s) compared to natural alginate. Palmitoylated alginate beads revealed 99% enantiomeric selectivity (ee) in production of L (+) lactic acid. Metabolite analysis during fermentation indicated low by-product (acetic acid, propionic acid and ethanol) formation on repeated batch fermentation with functionalized immobilized microbial cells. The scanning electron microscopic studies showed dense entrapped microbial cell biomass in modified immobilized beads compared to native alginate. Thus the methodology has great importance in large-scale production of optically pure lactic acid.
Item Type: | Article |
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Source: | Copyright of this article belongs to Springer. |
Keywords: | Alginate; Functionalization; Immobilization; Lactic Acid; Lactobacillus delbrueckii; Optical Purity |
ID Code: | 62800 |
Deposited On: | 24 Sep 2011 04:52 |
Last Modified: | 24 Sep 2011 04:52 |
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