Chatterjee, D. ; Mandal, C. ; Sarkar, P. K. (1987) Development and characterization of five monoclonal antibodies against neuronal cell surface antigens- evaluation of their use in cell separation by affinity chromatography Journal of Neuroimmunology, 15 (3). pp. 251-262. ISSN 0165-5728
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0165-5728(87)90120-2
Abstract
Five monoclonal antibodies directed against rat neuronal cell surface proteins have been isolated and characterized following fusion of mouse SP2/O-Ag-14 myeloma cells and spleen cells from BALB/c mice immunized with neuron-specific membrane proteins. The antibodies displayed cell specificity in binding and elicited precipitation of different polypeptides from total neuronal cell surface proteins. These antibodies are of potential use in the isolation of a neuronal subpopulation by immunoaffinity chromatography. The work represents the application of hybridoma technology for the isolation of specific types of neuronal populations.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Monoclonal Antibody; Immunoaffinity Chromatography; Neuronal Subpopulation |
| ID Code: | 61680 |
| Deposited On: | 15 Sep 2011 12:21 |
| Last Modified: | 15 Sep 2011 12:21 |
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