Binding of receptor-hydrocortisone complexes to isolated nuclei from embryonic neural retina cells

Abstract

Hydrocortisone (HC) induces glutamine synthetase in the embryonic chick neural retina. The binding of cytoplasmic receptor-hydrocortisone (R-HC) complexes to isolated retina nuclei has been studied in a cell-free system. Optimal conditions, specificity and quantitative aspects of binding were determined. The isolated nuclei retained binding specificity for the R-HC complex prepared from retina cytosol. Free HC, estradiol-receptor complexes from retina cytosol and HC-receptor complexes from mouse brain cytosol or from chick serum did not bind to the nuclei. Assuming monovalency of the binding sites, the number of nuclear acceptor sites per retina cell for the R-HC complex was estimated to be in the range of 1500. These sites were resistant to RNAse but sensitive to DNAse.