Swaminathan, Chittoor P. ; Surolia, Namita ; Surolia, Avadhesha (1998) Role of Water in the specific binding of mannose and mannooligosaccharides to concanavalin A Journal of the American Chemical Society, 120 (21). pp. 5153-5159. ISSN 0002-7863
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Official URL: http://pubs.acs.org/doi/abs/10.1021/ja9733696
Related URL: http://dx.doi.org/10.1021/ja9733696
Abstract
We report here, the first solution state evidence for the role of water molecules in the specific interaction of carbohydrates with a legume lectin, concanavalin A. Concanavalin A from Canavalia ensiformis is a protein containing 237 amino acid residues with each monomer possessing one sugar binding site as well as sites for transition-metal ions, Mn2+ and Ca2+. The lectin binds specifically to α -anomers of monosaccharides, D-glucopyranoside and D-mannopyranoside, and recognizes the trimannosidic core of N-linked glycoproteins, 3,6-di-O-(α -D-mannopyranosyl)-α -D-mannopyranoside with high specificity, which constitutes the minimum carbohydrate epitope that completely fills the sugar binding site. Sensitive isothermal titration microcalorimetry coupled with osmotic stress strategy on concanavalin A was used to dissect out the differential involvement of water molecules in the recognition of the branched trimannoside (3,6-di-O-(α -D-mannopyranosyl)-α -D-mannopyranoside), the individual dimannosidic arms (3-O-(α -D-mannopyranosyl)-α -D-mannopyranoside and 6-O-(α -D-mannopyranosyl)-α -D-mannopyranoside) as well as the monomer unit, D-mannopyranoside. The specific binding of concanavalin A to different sugars, is accompanied by differential uptake of water molecules during the binding process. These results not only complement the X-ray crystallographic studies of legume lectin-sugar complexes displaying structurally conserved water molecules mediating the specific ligation of the sugars with the corresponding sites in the binding pocket but also provide a rationale for the observed compensatory behavior of enthalpies with entropies in lectin-sugar interactions.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Chemical Society. |
ID Code: | 61584 |
Deposited On: | 15 Sep 2011 12:45 |
Last Modified: | 03 Oct 2011 14:16 |
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