Dasgupta, Indranil ; Das, Bijan K. ; Nath, Partha S. ; Mukhopadhyay, Sankar ; Niazi, F. R. ; Varma, Anupam (1996) Detection of rice tungro bacilliform virus in field and glasshouse samples from India using the polymerase chain reaction Journal of Virological Methods, 58 (1-2). pp. 53-58. ISSN 0166-0934
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0166-0934(95)01987-1
Abstract
Rice tungro bacilliform virus (RTBV) together with rice tungro spherical virus (RTSV) is the causal agent for the rice tungro disease. A rapid technique was developed to detect RTBV DNA in the crude extract of freshly collected leaf samples by polymerase chain reaction (PCR). This technique can detect the viral DNA in 1000-fold diluted leaf extract. Detection has been possible in samples stored upto 5 days after the collection. This technique may have wide application for the field diagnosis of RTBV infection.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Tungro; Detection; Polymerase Chain Reaction; Primers |
| ID Code: | 60483 |
| Deposited On: | 09 Sep 2011 03:39 |
| Last Modified: | 09 Sep 2011 03:39 |
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