Chattopadhyay, Samit ; Ghosh, Ranajit Kumar (1988) Localization of transfer RNA genes on the physical map of Vibrio eltor phage e4 genome Virology, 162 (2). pp. 337-345. ISSN 0042-6822
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0042-6822(88)90473-4
Abstract
Transfer RNAs were isolated from uninfected and phage e4-infected Vibrio eltor Mak 757 cells. These tRNAs were then aminoacylated with 3H-labeled amino acids and hybridized to DNA isolated from phage e4. Significant hybridization was observed only with tRNA isolated from phage e4-infected cells. Restriction enzyme digestion of phage e4 DNA followed by Southern blot using [32P]tRNA from infected cells revealed that tRNA genes were contained in a 3.4-kb Kpnl fragment. The tRNA genes were located on the physical map of the phage genome 19 kb from one of the termini.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 60252 |
| Deposited On: | 08 Sep 2011 12:12 |
| Last Modified: | 08 Sep 2011 12:12 |
Repository Staff Only: item control page
