Kachroo, P. ; Potnis, A. ; Chattoo, B. B. (1997) Transformation of the rice blast fungus Magnaporthe grisea to benomyl resistance World Journal of Microbiology and Biotechnology, 13 (2). pp. 185-187. ISSN 0959-3993
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Official URL: http://www.springerlink.com/content/g7v22105k28501...
Related URL: http://dx.doi.org/10.1023/A:1018589714357
Abstract
A transformation method based on a dominant selectable marker (benomyl resistance) was developed for the rice blast fungus Magnaporthe grisea. The heterologous gene for β-tubulin from Neurospora crassa (pBT3) was used to obtain benomyl-resistant M. grisea transformants at a frequency of 20 to 30/μg of DNA. Control transformations carried out with a plasmid conferring hygromycin resistance or a derivative of pBT3 containing a repetitive DNA sequence, yielded the same frequency of transformation as that of pBT3. Molecular analysis of the transformants indicated multiple integration of the vector DNA.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Benomyl Resistance; Magnaporthe grisea; Protoplast Transformation; Selectable Marker |
| ID Code: | 60113 |
| Deposited On: | 08 Sep 2011 09:58 |
| Last Modified: | 08 Sep 2011 09:58 |
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