Chauhan, Sanjay ; Iyengar, M. R. S. ; Chattoo, B. B. (1998) Factors influencing the production of penicillin V acylase by Chainia, a sclerotial Streptomyces Journal of Basic Microbiology, 38 (3). pp. 173-179. ISSN 0233-111X
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1...
Related URL: http://dx.doi.org/10.1002/(SICI)1521-4028(199807)38:3<173::AID-JOBM173>3.0.CO;2-J
Abstract
Aerobic culture of a Streptomyces was found to produce penicillin V acylase (PVA) (PA, EC-3.5.1.11) extracellularly. Strain characteristics recorded on various media and presence of mature sclerotia led to its identification as Chainia, a sclerotial Streptomyces. The Chainia acylase hydrolyzed penicillin V (Pen V) preferentially. Maximum conversion of Pen V to 6-aminopenicillanic acid (6-APA) was observed when the 48 hour old fermented broth was used as an enzyme source. Fermentation parameters and the culture condition of the strain was optimized for high acylase activity.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| ID Code: | 60102 |
| Deposited On: | 08 Sep 2011 09:59 |
| Last Modified: | 08 Sep 2011 09:59 |
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