Tendulkar, Sachin R. ; Gupta, Archna ; Chattoo, Bharat B. (2003) A simple protocol for isolation of fungal DNA Biotechnology Letters, 25 (22). pp. 1941-1944. ISSN 0141-5492
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Official URL: http://www.springerlink.com/content/j9j736766t4313...
Related URL: http://dx.doi.org/10.1023/B:BILE.0000003990.27624.04
Abstract
Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and for a quick screening of M. grisea transformants.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Genomic DNA; Magnaporthe Grisea; Microwave Treatment; PCR; RAPD |
| ID Code: | 60095 |
| Deposited On: | 08 Sep 2011 06:44 |
| Last Modified: | 08 Sep 2011 06:44 |
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