Arun, Narasimhan ; Mukhopadhyay, Sabyasachi ; Narayan, K. S. (2010) Monitoring intermediate states of bacteriorhodopsin monolayers using near-field optical microscopy Applied Optics, 49 (7). pp. 1131-1138. ISSN 1539-4522
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Official URL: http://www.opticsinfobase.org/abstract.cfm?URI=ao-...
Related URL: http://dx.doi.org/10.1364/AO.49.001131
Abstract
We demonstrate single- molecule- level features using near- field optical microscopy on bacteriorhodopsin (bR), a membrane protein that functions as a light- driven proton pump. The photophysical properties of bR are utilized in this imaging technique, using a combination of photoexcitation sources, to accurately identify the active regions and quantify the optical parameters. The studies of bR monolayers are carried out on inert quartz substrates as well as active conducting polymer (polyaniline) substrates. The substrate also plays an important role in the photocycle quantum efficiencies. We speculate on mechanisms governing the higher near- field absorption strength of bR molecules.
Item Type: | Article |
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Source: | Copyright of this article belongs to Optical Society of America. |
ID Code: | 60074 |
Deposited On: | 08 Sep 2011 10:09 |
Last Modified: | 08 Sep 2011 10:09 |
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