Pazin, Michael J. ; Bhargava, Purnima ; Peter Geiduschek, E. ; Kadonaga, James T. (1997) Nucleosome mobility and the maintenance of nucleosome positioning Science, 276 (5313). pp. 809-812. ISSN 0036-8075
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Official URL: http://www.sciencemag.org/content/276/5313/809.abs...
Related URL: http://dx.doi.org/10.1126/science.276.5313.809
Abstract
To study nucleosome mobility and positioning, the R3lac repressor was used with an adenosine triphosphate (ATP)-dependent chromatin assembly system to establish the positioning of five nucleosomes, with one nucleosome located between two R3 lac operators. When R3 protein was dissociated from DNA with isopropyl β-d-thiogalactopyranoside, the R3-induced nucleosome positions remained unchanged for at least 60 minutes in the absence of ATP but rearranged within 15 minutes in the presence of ATP. These results suggest that nucleosomes are dynamic and mobile rather than static and that a DNA binding factor is continuously required for the maintenance of nucleosome positioning.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to American Association for the Advancement of Science. |
| ID Code: | 60002 |
| Deposited On: | 08 Sep 2011 10:12 |
| Last Modified: | 08 Sep 2011 10:12 |
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