Vijay Raghavan, K. ; Mayeda, Carol ; Meyerowitz, Elliot M. (1988) The action of the I(1)npr-1+ locus on the Drosophila glue gene Sgs-3 is cell-autonomous Journal of Genetics, 67 (3). pp. 141-150. ISSN 0022-1333
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Official URL: http://www.ias.ac.in/jarch/jgenet/67/141.pdf
Related URL: http://dx.doi.org/10.1007/BF02927825
Abstract
The 2B5 chromosomal locus inDrosophila contains a gene, I(1)npr-1+, whose product required intrans for the expression of the larval salivary gland-specific geneSgs-3. We have addressed the question as to whether this factor acts in a cell-autonomous manner or not. This was made possible by the use of a transformant strain that makes an Sgs-3-E. coli β-galactosidase (lacZ) fusion protein, under the control of an Sgs-3 promoter, allowing the cellular examination of gene expression by a histochemical assay for enzyme activity. Using genetic methods, larvae that were mosaic for the loss of function mutationl (l) npr-1 were generated. The expression of the Sgs-3-lacZ fusion gene was assayed histochemically in such larvae. Our results strongly indicate a cell-autonomous requirement for the product ofl(1) npr-1+. This is in contrast to another factor, the hormone ecdysterone, which is also required for Sgs-3 expression, but acts in a non-autonomous manner.
Item Type: | Article |
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Source: | Copyright of this article belongs to Indian Academy of Sciences. |
Keywords: | Drosophila; Sgs-3; L(1)npr-1; Trans-acting; Glue; Ecdysterone |
ID Code: | 58621 |
Deposited On: | 31 Aug 2011 11:57 |
Last Modified: | 18 May 2016 09:30 |
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