Purohit, S. D. ; Raghuvanshi, S. ; Tyagi, A. K. (2007) Biolistic-mediated DNA delivery and transient expression of GUS in hypocotyls of feronia limonia L.- A fruit tree Indian Journal of Biotechnology, 6 (4). pp. 504-507. ISSN 0972-5849
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Abstract
Feronia limonia L. (Rutaceae) is an important fruit tree of arid horticulture. To be able to introduce genes of interest for its improvement, biolistic-mediated DNA delivery method has been standardized. The hypocotyls explants were bombarded with plasmid pBI121 having gus reporter gene driven by CaMV 35S promoter and npt II under control of nos promoter as selectable marker. The best transient expression of GUS was recorded when tungsten particles coated with DNA were bombarded at a disc pressure of 1350 and 1100 psi from a distance of 9 cm. Some of the bombarded explants are being maintained to obtain viable regenerates for their analyses.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| Keywords: | Biolistic; Feronia Limonia; Transient Expression |
| ID Code: | 57999 |
| Deposited On: | 30 Aug 2011 10:22 |
| Last Modified: | 30 Aug 2011 10:22 |
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