Tyagi, S. ; Sporlein, B. ; Tyagi, A. K. ; Herrmann, R. G. ; Koop, H. U. (1989) PEG- and electroporation-induced transformation in Nicotiana tabacum: influence of genotype on transformation frequencies Theoretical and Applied Genetics (TAG), 78 (2). pp. 287-292. ISSN 0040-5752
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Official URL: http://www.springerlink.com/content/u335h813h6u170...
Related URL: http://dx.doi.org/10.1007/BF00288813
Abstract
Experimental parameters for direct gene transfer with recombinant DNA encoding neomycin phosphotransferase II (NPTII) under control of eukaryotic expression signals were established. The introduced gene was shown by the growth of transformants on media containing kanamycin, by genomic blotting and by assaying NPTII activity. Leaf protoplasts from three green genotypes of varieties xanthii and petit havanna, and from four plastome-encoded albino genotypes of Nicotiana tabacum were analyzed with respect to cell division kinetics and yield of kanamycin-tolerant colonies after direct gene transfer. No clear correlation was found between the time of onset of cell division and transformation frequency.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Direct Gene Transfer; Electroporation; Genotype Influence; Kanamycin Resistance; Nicotiana tabacum |
| ID Code: | 57976 |
| Deposited On: | 30 Aug 2011 10:14 |
| Last Modified: | 30 Aug 2011 10:14 |
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