Nieh, M.-P. ; Harroun, T. A. ; Raghunathan, V. A. ; Glinka, C. J. ; Katsaras, J. (2004) Spontaneously formed monodisperse biomimetic unilamellar vesicles: the effect of charge, dilution, and time Biophysical Journal, 86 (4). pp. 2615-2629. ISSN 0006-3495
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Official URL: http://www.cell.com/biophysj/retrieve/pii/S0006349...
Related URL: http://dx.doi.org/10.1016/S0006-3495(04)74316-7
Abstract
Using small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca2+) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration ≤1wt%), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be ∼20%. The stability of the Ca2+- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, <Ro>) were found to be insensitive to the lipid concentration (Clp). However, doping the dimyristoyl/dihexanoyl system with both Ca2+ and DMPG resulted in ULVs whose <Ro> was found to be Clp dependent. The <Ro> of DMPG-doped ULVs remained unchanged over an extended period of time (at least 4 days), a good indication of their stability.
Item Type: | Article |
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Source: | Copyright of this article belongs to Biophysical Society. |
ID Code: | 57514 |
Deposited On: | 27 Aug 2011 12:26 |
Last Modified: | 18 May 2016 08:53 |
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