Phenoxazine functionalized, exfoliated graphite based electrodes for NADH oxidation and ethanol biosensing

Ramesh, P. ; Sivakumar, P. ; Sampath, S. (2003) Phenoxazine functionalized, exfoliated graphite based electrodes for NADH oxidation and ethanol biosensing Electroanalysis, 15 (23-24). pp. 1850-1858. ISSN 1040-0397

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/elan.20...

Related URL: http://dx.doi.org/10.1002/elan.200302762

Abstract

Exfoliated graphite (EG) particles covalently functionalized with phenoxazine-based molecules have been used to prepare bulk-modified electrodes. The electrodes are of two types: 1) binder-less covalently modified EG pellets and 2) sol-gel derived composites of silicate and modified EG particles. The covalent modification is confirmed by infrared spectroscopy. The electrochemistry of attached molecules has been carried out to decipher the catalytic activity of immobilized phenoxazines towards NADH oxidation. Fast response time of about 4-6 seconds and a low detection limit of 20 µM have been achieved using these electrodes. The sensor is found to yield a linear range of current response versus concentration between 0.02 and 1 mM of NADH. Biosensing in presence of alcohol dehydrogenase enzyme and NAD+ shows a linear response between 1 and 13 mM and the response time for alcohol sensing is found to be 20-30 s. These electrodes are found to be very stable during operation and can be stored without any deterioration over a period of several months.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:NADH oxidation; Sol-gel; Binderless Electrodes; Ethanol Biosensing; Exfoliated Graphite
ID Code:57340
Deposited On:26 Aug 2011 08:00
Last Modified:26 Aug 2011 08:00

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