Structure and activity of OK-GC: a kidney receptor guanylate cyclase activated by guanylin peptides

London, Roslyn M. ; Eber, Sammy L. ; Visweswariah, Sandhya S. ; Krause, William J. ; Forte, Leonard R. (1999) Structure and activity of OK-GC: a kidney receptor guanylate cyclase activated by guanylin peptides American Journal of Physiology - Renal Physiology, 276 (6). F882-F891. ISSN 0363-6127

Full text not available from this repository.

Official URL:


Uroguanylin, guanylin, and lymphoguanylin are small peptides that activate renal and intestinal receptor guanylate cyclases (GC). They are structurally similar to bacterial heat-stable enterotoxins (ST) that cause secretory diarrhea. Uroguanylin, guanylin, and ST elicit natriuresis, kaliuresis, and diuresis by direct actions on kidney GC receptors. A 3,762-bp cDNA characterizing a uroguanylin/guanylin/ST receptor was isolated from opossum kidney (OK) cell RNA/cDNA. This kidney cDNA (OK-GC) encodes a mature protein containing 1,049 residues sharing 72.4-75.8% identity with rat, human, and porcine forms of intestinal GC-C receptors. COS or HEK-293 cells expressing OK-GC receptor protein were activated by uroguanylin, guanylin, or ST13 peptides. The 3.8-kb OK-GC mRNA transcript is most abundant in the kidney cortex and intestinal mucosa, with lower mRNA levels observed in urinary bladder, adrenal gland, and myocardium and with no detectable transcripts in skin or stomach mucosa. We propose that OK-GC receptor GC participates in a renal mechanism of action for uroguanylin and/or guanylin in the physiological regulation of urinary sodium, potassium, and water excretion. This renal tubular receptor GC may be a target for circulating uroguanylin in an endocrine link between the intestine and kidney and/or participate in an intrarenal paracrine mechanism for regulation of kidney function via the intracellular second messenger, cGMP.

Item Type:Article
Source:Copyright of this article belongs to The American Physiological Society.
Keywords:Guanosine 3',5'-cyclic Monophosphate; Uroguanylin; OK Cells
ID Code:56960
Deposited On:25 Aug 2011 09:15
Last Modified:25 Aug 2011 09:15

Repository Staff Only: item control page