Visweswariah, Sandhya S. ; Shanthi, G. ; Balganesh, T. S. (1992) Interaction of heat-stable enterotoxins with human colonie (T84) cells: modulation of the activation of guanylyl cyclase Microbial Pathogenesis, 12 (3). pp. 209-218. ISSN 0882-4010
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0882-4010(92)90055-S
Abstract
Heat-stable enterotoxins (ST) activate guanylyl cyclase in T84 cells, rapidly and specifically. Activation is monitored by cGMP production and occurs at lower concentrations of ST than required for eliciting fluid accumulation in suckling mice. Atrial natriuretic factor (ANF) neither activates guanylyl cyclase nor modulates the response to ST in T84 cells, indicating the absence of receptors for ANF on T84 cells. Monitoring the production of cGMP under conditions known to alter fluid accumulation in suckling mice is an accurate and quantifiable assay of ST activity and its interaction with the receptor. STs produced by Escherichia coli, Vibrio cholerae non-01 and Yersinia enterocolitica individually produce elevated levels of cGMP in T84 cells, but to differing extents, suggesting that this model system can be used to elucidate the different events of ST-receptor interactions at the molecular level.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Diarrhoea; Stable Toxin; T84 Cells; Guanylyl Cyclase; Cyclic GMP |
| ID Code: | 56952 |
| Deposited On: | 25 Aug 2011 09:15 |
| Last Modified: | 25 Aug 2011 09:15 |
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