Pain, Debkumar ; Surolia, Avadhesha (1980) A new approach for quantitation of receptors and their ligands Journal of Molecular Biology, 143 (4). pp. 419-425. ISSN 0022-2836
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0022-2836(80)90221-1
Abstract
A novel and sensitive method for quantitating receptors and their ligands is described, using the calf uterine estrogen receptor and estradiol as a model system. When ligand conjugated to malate dehydrogenase is incubated with the estrogen receptor, the enzyme is inhibited proportionately to the concentration of receptor. However, receptor saturated with free ligand has almost no effect on the ligand-conjugated enzyme. The assay can detect as little as three femtomoles of receptor and one femtomole of estradiol and can, in principle, be applied to any receptor-ligand interaction.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 56681 |
| Deposited On: | 25 Aug 2011 10:23 |
| Last Modified: | 25 Aug 2011 10:23 |
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