Sharma, Shailendra Kumar ; Kumar, Gyanendra ; Kapoor, Mili ; Surolia, Avadhesha (2008) Combined effect of epigallocatechin gallate and triclosan on enoyl-ACP reductase of Mycobacterium tuberculosis Biochemical and Biophysical Research Communications, 368 (1). pp. 12-17. ISSN 0006-291X
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.bbrc.2007.10.191
Abstract
Among the various inhibitors known for enoyl-acyl carrier protein (ACP) reductases, triclosan and green tea catechins are two promising candidates. In the present study, we show, for the first time that epigallocatechin gallate (EGCG), a major component of green tea catechins, inhibits InhA, the enoyl-ACP reductase of Mycobacterium tuberculosis with an IC50 of 17.4 μM. EGCG interferes with the binding of NADH to InhA. We also demonstrate that EGCG increased the inhibitory activity of triclosan towards InhA and vice versa. Direct binding assay using [3H]EGCG and fluorescence titration assay support the spectrophotometric/kinetic inhibition data. The biochemical data has been explained by docking simulation studies.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Mycobacterium tuberculosis; Tuberculosis; InhA; Enoyl Reductase; FAS I; FAS II; Tea Catechins; Epigallocatechin Gallate; EGCG; Triclosan; Fluorescence Titration; Scatchard Plot |
| ID Code: | 56654 |
| Deposited On: | 25 Aug 2011 10:26 |
| Last Modified: | 25 Aug 2011 10:26 |
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