Cloning, expression, purification, crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis

Selvaraj, M. ; Singh, N. S. ; Roy, Siddhartha ; Sangeetha, R. ; Varshney, Umesh ; Vijayan, M. (2006) Cloning, expression, purification, crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis Acta Crystallographica Section F, 62 (9). pp. 913-915. ISSN 1744-3091

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Official URL: http://onlinelibrary.wiley.com/doi/10.1107/S174430...

Related URL: http://dx.doi.org/10.1107/S1744309106031125

Abstract

Peptidyl-tRNA hydrolase catalyses the cleavage of the ester link between the peptide and the tRNA in peptidyl-tRNAs that, for various reasons, have dropped off the translating ribosomes. This enzyme from Mycobacterium tuberculosis has been crystallized in three related but distinct forms: P212121, unit-cell parameters a = 36.30, b = 61.85, c = 73.97 Å, P21, a = 35.83, b = 73.79, c = 59.79 Å, β = 92.3°, and P212121, a = 35.84, b = 57.06, c = 72.59 Å. X-ray data have been collected from all three forms.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Peptidyl-tRNA Hydrolase; Mycobacterium tuberculosis;
ID Code:56238
Deposited On:23 Aug 2011 11:56
Last Modified:16 Jul 2012 05:32

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