Swarup, G. ; Kamatkur, S. ; Radha, V. ; Rema, V. (1991) Molecular cloning and expresion of a protein-tyrosine phosphatase showing homology with transcription factors Fos and Jun FEBS Letters, 280 (1). pp. 65-69. ISSN 0014-5793
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0014-5793(91)80205-H
Abstract
A cDNA clone coding for a protein-tyrosine phosphatase (PTPase) was isolated from a rat spleen cDNA library. Nucleotide sequence of the clone showed an open reading frame coding for a polypeptide of 363 amino acids. Expression of this clone in E. coli in an expression vector showed PTPase activity. The non-catalytic region of this PTPase located at the carboxy terminus shows homology with the basic domains of transcription factors Fos and Jun. Northern blot analysis showed that a 1.7 kb transcript was present in many tissues and cells, the highest level being in macrophages. This PTPase is a rat homolog of human T-cell PTPase although it shows 3 large deletions in the carboxy terminal non-catalytic region.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
| Keywords: | Protein-tyrosine Phosphatase; cDNA Cloning; Gene Expression; Transcription Factor |
| ID Code: | 55936 |
| Deposited On: | 19 Aug 2011 07:54 |
| Last Modified: | 18 Jan 2012 04:45 |
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