Hesselgesser, J. ; Chitnis, C. E. ; Miller, L. H. ; Yansura, D. G. ; Simmons, L. C. ; Fairbrother, W. J. ; Kotts, C. ; Wirth, C. ; Gillece-Castro, B. L. ; Horuk, R. (1995) A mutant of melanoma growth stimulating activity does not activate neutrophils but blocks erythrocyte invasion by malaria Journal of Biological Chemistry, 270 (19). pp. 11472-11476. ISSN 0021-9258
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Official URL: http://www.jbc.org/content/270/19/11472.abstract
Abstract
Alanine scanning mutagenesis of the charged amino acids of melanoma growth stimulating activity (MGSA) was used to identify specific residues that are involved in binding to the human erythrocyte Duffy antigen/chemokine receptor (DARC) and to the type B interleukin-8 receptor (IL-8RB) on neutrophils. Receptor binding and biological studies with the alanine scan mutants of MGSA demonstrate that MGSA binds to DARC and the IL-8RB through distinct binding regions. One of the MGSA mutants, E6A, binds to human erythrocytes and is able to inhibit malaria invasion as efficiently as wild type MGSA but has a severely reduced ability to bind to or signal through the IL-8RB. Mutant chemokines like E6A could prove to be useful therapeutically for the design of receptor blocking drugs that inhibit erythrocyte invasion by Plasmodium vivax malaria.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Society for Biochemistry and Molecular Biology. |
ID Code: | 5570 |
Deposited On: | 19 Oct 2010 11:53 |
Last Modified: | 16 May 2016 16:03 |
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