Lemieux, Raymond U. ; Du, Ming-Hui ; Spohr, Ulrike ; Acharya, Shreeta ; Surolia, Avadhesha (1994) Molecular recognition XIII. The binding of the H-type 2 human blood group determinant by a winged bean (Psophocarpus tetragonolobus) acidic lectin Canadian Journal of Chemistry, 72 (1). pp. 158-163. ISSN 0008-4042
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Official URL: http://www.nrcresearchpress.com/doi/abs/10.1139/v9...
Related URL: http://dx.doi.org/10.1139/v94-024
Abstract
The winged bean acidic lectin (WBA II) binds the H-type 2 human blood group related trisaccharide (α-L-Fuc-(1c → 2b)-β-D-Gal-(1b → 4a)-β-D-GlcNAc-OMe, 1). Interactions that importantly contribute to the specificity of the complex formation are provided by CH2-6b, OH-4b, OH-3b, and OH-2c of 1. On the basis of the relative activities of the monodeoxy and mono-O-methyl derivatives of 1, the hydroxyl groups at the 3a, 6a, and 4c positions become located at the periphery of the combining site, whereas the CH3O-1a, NHAc-2a, and CH3-5c groups are more remote from the protein surface and fully exposed to bulk water. Whereas the WBA II lectin marginally recognizes the H-type 1 related trisaccharide (α-L-Fuc-(1c → 2b)-β-D-Gal-(1b → 3a)-β-D-GlcNAc-OMe, 23), the de-N-acetyl derivative (24) is bound 3.4 times more strongly than 1. This and other results, which are related to changes in interaction between the ligand and the apron of the combining site, are attributed to changes in hydration that lead to enthalpy-entropy compensation. In certain cases, the interactions are found to stabilize the complex.
Item Type: | Article |
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Source: | Copyright of this article belongs to NRC Research Press. |
ID Code: | 55385 |
Deposited On: | 18 Aug 2011 09:11 |
Last Modified: | 18 Aug 2011 09:11 |
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