Kaur, Rupinderjeet ; Sachdevaa, Geetanjali ; Vrati, Sudhanshu (2002) Plasmid DNA immunization against Japanese encephalitis virus: immunogenicity of membrane-anchored and secretory envelope protein The Journal of Infectious Diseases, 185 (1). pp. 1-12. ISSN 0022-1899
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Official URL: http://jid.oxfordjournals.org/content/185/1/1.abst...
Related URL: http://dx.doi.org/10.1086/338015
Abstract
Plasmid DNA synthesizing membrane-anchored or secretory Japanese encephalitis virus (JEV) envelope (E) protein and premembrane protein was delivered to mice by intramuscular injection or gene gun. Intramuscular plasmid immunization induced anti-E antibody responses similar to those associated with commercial JEV vaccine. The gene gun induced less antibody response. The 2 forms of the E protein induced similar antibody titers when administered by the same delivery mode. Both plasmids generated high titers of JEV-neutralizing antibodies, although the titers were lower than those induced by the vaccine. Intramuscular DNA immunization induced T helper 1 (Th1) immune responses, and the gene gun induced Th2 responses. Compared with secretory E protein, the membrane-anchored protein heavily skewed the immune response toward either Th1 or Th2, depending on the route of immunization. In an intracerebral JEV challenge model, plasmid-immunized mice had ~60% protection; this was not affected by the form of the E protein or by immunization route.
Item Type: | Article |
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Source: | Copyright of this article belongs to University of Chicago Press. |
ID Code: | 55144 |
Deposited On: | 17 Aug 2011 11:32 |
Last Modified: | 17 Aug 2011 11:32 |
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