Expression of Japanese encephalitis virus envelope protein in transgenic tobacco plants

Appaiahgari, Mohan Babu ; Abdin, M. Z. ; Bansal, K. C. ; Vrati, Sudhanshu (2009) Expression of Japanese encephalitis virus envelope protein in transgenic tobacco plants Journal of Virological Methods, 162 (1-2). pp. 22-29. ISSN 0166-0934

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.jviromet.2009.07.002

Abstract

The virus envelope (E) protein of Japanese encephalitis virus induces virus-neutralizing antibodies and is therefore a potential vaccine antigen. In a mammalian system, co-expression of another viral structural protein prM is necessary for proper expression and folding of E protein. Transgenic tobacco plants were produced carrying JEV cDNA encoding prM and E proteins under the control of the CaMV 35S promoter. E protein, however, was not detectable in these plants. In vitro translation studies showed that the presence of the prM sequence inhibited transgene expression in the plant system. Accordingly, JEV E protein could be expressed in transgenic tobacco plants only without the prM protein.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Flavivirus; Vaccine; Transcription; Translation; Protein Processing; RNA
ID Code:55062
Deposited On:18 Aug 2011 07:49
Last Modified:18 Aug 2011 07:49

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