Appaiahgari, Mohan Babu ; Abdin, M. Z. ; Bansal, K. C. ; Vrati, Sudhanshu (2009) Expression of Japanese encephalitis virus envelope protein in transgenic tobacco plants Journal of Virological Methods, 162 (1-2). pp. 22-29. ISSN 0166-0934
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.jviromet.2009.07.002
Abstract
The virus envelope (E) protein of Japanese encephalitis virus induces virus-neutralizing antibodies and is therefore a potential vaccine antigen. In a mammalian system, co-expression of another viral structural protein prM is necessary for proper expression and folding of E protein. Transgenic tobacco plants were produced carrying JEV cDNA encoding prM and E proteins under the control of the CaMV 35S promoter. E protein, however, was not detectable in these plants. In vitro translation studies showed that the presence of the prM sequence inhibited transgene expression in the plant system. Accordingly, JEV E protein could be expressed in transgenic tobacco plants only without the prM protein.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Flavivirus; Vaccine; Transcription; Translation; Protein Processing; RNA |
ID Code: | 55062 |
Deposited On: | 18 Aug 2011 07:49 |
Last Modified: | 18 Aug 2011 07:49 |
Repository Staff Only: item control page