Immunogenicity and protective efficacy of the E. coli-expressed domain III of Japanese encephalitis virus envelope protein in mice

Alka, ; Bharati, Kaushik ; Malik, Y. P. S. ; Vrati, Sudhanshu (2007) Immunogenicity and protective efficacy of the E. coli-expressed domain III of Japanese encephalitis virus envelope protein in mice Medical Microbiology and Immunology, 196 (4). pp. 227-231. ISSN 0300-8584

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Official URL: http://www.springerlink.com/content/l320h054221506...

Related URL: http://dx.doi.org/10.1007/s00430-007-0043-4

Abstract

Domain III of Japanese encephalitis virus (JEV) envelope protein (E-DIII) was synthesized in E. coli as a fusion protein containing maltose-binding protein (MBP-E-DIII) or six contiguous histidine residues (His-E-DIII) at its N-terminus. MBP-E-DIII was found both in the soluble as well as the insoluble fraction of the bacterial lysate, while His-E-DIII was found exclusively in the inclusion bodies. These purified proteins were examined in mice for their immunogenicity in presence of an aluminium hydroxide based-adjuvant Alhydrogel and Freund's adjuvant. While both proteins generated anti-JEV antibodies that neutralized JEV activity in vitro, His-E-DIII generated higher antibody titers than MBP-E-DIII. Mice immunized with His-E-DIII in presence of Alhydrogel generated antibody titers similar to those induced by the commercial vaccine and protected mice against lethal JEV challenge.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Neutralization; Enzyme-linked Immunosorbent Assay; Adjuvant
ID Code:55041
Deposited On:18 Aug 2011 07:35
Last Modified:18 Aug 2011 07:35

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