Upadhyay, Santosh Kumar ; Mishra, Manisha ; Singh, Harpal ; Ranjan, Amol ; Chandrashekar, Krishnappa ; Verma, Praveen Chandra ; Singh, Pradhyumna Kumar ; Tuli, Rakesh (2010) Interaction of Allium sativum leaf agglutinin with midgut brush border membrane vesicles proteins and its stability in Helicoverpa armigera Proteomics, 10 (24). pp. 4431-4440. ISSN 1615-9853
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/pmic.20...
Related URL: http://dx.doi.org/10.1002/pmic.201000152
Abstract
Allium sativum leaf agglutinin (ASAL) binds to several proteins in the midgut of Helicoverpa armigera and causes toxicity. Most of these were glycosylated. Six ASAL-binding proteins were selected for identification. PMF and MS/MS data showed their similarity with midgut aminopeptidase APN2, polycalins and alkaline phosphatase of H. armigera, cadherin-N protein (partial AGAP009726-PA) of Acyrthosiphon pisum, cytochrome P450 (CYP315A1) of Manduca sexta and alkaline phosphatase of Heliothis virescens. Some of the ASAL-binding midgut proteins were similar to the larval receptors responsible for the binding of δ-endotoxin proteins of Bacillus thuringiensis. Galanthus nivalis agglutinin also interacted with most of the ASAL-binding proteins. The ASAL showed resistance to midgut proteases and was detected in the larval hemolymph and excreta. Immunohistochemical staining revealed the presence of ASAL in the body tissue also.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| Keywords: | Allium sativum Leaf Agglutinin; Animal Proteomics; Bacillus thuringiensis; Glycosylated; Helicoverpa armigera; Midgut |
| ID Code: | 54616 |
| Deposited On: | 12 Aug 2011 07:06 |
| Last Modified: | 12 Aug 2011 07:06 |
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