Shankar, Sandeep ; Tyagi, Anil K. (1993) MchAI and MchAII, two class-II restriction endonucleases from Mycobacterium chelonei Gene, 132 (1). pp. 119-123. ISSN 0378-1119
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0378-1119(93)90523-6
Abstract
We have purified and characterized two class-II restriction endonucleases from the saprophyte Mycobacterium chelonei AMB 82. MchAI was an isoschizomer of NotI recognizing and cleaving at 5'-GC/GGCCGC. MchAI did not require Triton X-100 for activity and was fully active at concentrations over 10-200 mM KCl and MgCl2. MchAII was an isoschizomer of HaeII recognizing and cleaving at 5'-GG/CC. MchAII was active in 10 mM Tris-HCl pH 8.0 containing MgCl2 at 10-200 mM, was strongly inhibited by NaCl at concentrations higher than 10 mM and was inactive at KCl concentrations lower than 250 mM.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Recombinant DNA; Sequence specificity; NotI and HaeIII isoschizomers: Mycobacteria |
| ID Code: | 54551 |
| Deposited On: | 12 Aug 2011 07:09 |
| Last Modified: | 12 Aug 2011 07:09 |
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