Sonar, Sanjay M. ; Singh, Anil K. (1991) Location of lysine-129 and lysine-40/41 with respect to retinylidene chromophore in bacteriorhodopsin Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1076 (2). pp. 239-244. ISSN 0167-4838
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0167-4838(91)90273-3
Abstract
Fluorescence properties of fluorescamine-modified bacteriorhodopsin (BR) have been studied. BR reacts with fluorescamine (1:2 ratio) to give protein (FLBR-I) modified at Lys-129. By making use of citraconic anhydride as a masking reagent, fluorescamine modification of Lys-40/41 in BR has been achieved. Forster's resonance energy transfer studies indicate that the distance between FL-Lys-129 and retinylidene chromophore is 11Å, whereas that between FL-Lys-40/41 and retinylidene chromophore is 24 Å. These measured distances have been analysed in terms of BR structure.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Bacteriorhodopsin; Chemical Modification; Fluorescamine; Fluorescence; Forster Resonance Energy Transfer; (H. halobium) |
| ID Code: | 54115 |
| Deposited On: | 11 Aug 2011 11:44 |
| Last Modified: | 11 Aug 2011 11:44 |
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