Sankara Rao, K. ; Venkateswara, R. (1985) Tissue culture of forest trees: clonal multiplication of Eucalyptus grandis L. Plant Science, 40 (1). pp. 51-55. ISSN 0168-9452
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0168-9452(85)90162-1
Abstract
Axillary shoot proliferation was obtained using explants of Eucalyptus grandis L. juvenile and mature stages on a defined medium. Murashige and Skoog medium (MS) supplemented with benzyladenine (BA), naphthalene acetic acid (NAA) and additional thiamine. Excised shoots were induced to root on a sequence of three media: (1) White's medium containing indoleacetic acid (IAA), NAA and indole butyric acid; (IBA), (2) half-strength MS medium with charcoal and (3) half-strength MS liquid medium. The two types of explants differed in rooting response, with juvenile-derived shoots giving 60% rooting and adult-derived ones only 35%. Thus, the factors limiting cloning of selected trees in vitro are determined to be those controlling rooting of shoots in E. grandis.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Eucalyptus grandis L.; Clonal Multiplication; Axillary Shoot Proliferation; Tissue Cultures |
| ID Code: | 54032 |
| Deposited On: | 11 Aug 2011 12:05 |
| Last Modified: | 11 Aug 2011 12:05 |
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