Sadhale, P. P. ; Woychik, N. A. (1994) C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7 Molecular and Cellular Biology, 14 (9). pp. 6164-6170. ISSN 0270-7306
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Official URL: http://mcb.asm.org/cgi/content/abstract/14/9/6164
Abstract
We identified a partially sequenced Saccharomyces cerevisiae gene which encodes a protein related to the S. cerevisiae RNA polymerase II subunit, RPB7. Several lines of evidence suggest that this related gene, YKL1, encodes the RNA polymerase III subunit C25. C25, like RPB7, is present in submolar ratios, easily dissociates from the enzyme, is essential for cell growth and viability, but is not required in certain transcription assays in vitro. YKL1 has ABF-1 and PAC upstream sequences often present in RNA polymerase subunit genes. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobility of the YKL1 gene product is equivalent to that of the RNA polymerase III subunit C25. Finally, a C25 conditional mutant grown at the nonpermissive temperature synthesizes tRNA at reduced rates relative to 5.8S rRNA, a hallmark of all characterized RNA polymerase III mutants.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Society for Microbiology. |
ID Code: | 54005 |
Deposited On: | 11 Aug 2011 11:57 |
Last Modified: | 11 Aug 2011 11:57 |
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