Cloning, expression, purification, crystallization and preliminary X-ray studies of the mannose-binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis

Patra, D. ; Sharma, A. ; Chandran, D. ; Vijayan, M. (2011) Cloning, expression, purification, crystallization and preliminary X-ray studies of the mannose-binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis Acta Crystallographica Section F, 67 . pp. 596-599. ISSN 1744-3091

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Official URL: http://scripts.iucr.org/cgi-bin/paper?ub5018

Related URL: http://dx.doi.org/10.1107/S1744309111009547

Abstract

The mannose-binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis has been cloned, expressed, purified and crystallized and the crystals have been characterized using X-ray diffraction. The Matthews coefficient suggests the possibility of two lectin domains in the triclinic cell. The amino-acid sequence of the domain indicates structural similarity to well characterized β-prism II fold lectins.

Item Type:Article
Source:Copyright of this article belongs to International Union of Crystallography.
Keywords:Bacterial Lectins; Mycobacterium smegmatis; β-prism II Fold; Sugar Binding
ID Code:53861
Deposited On:10 Aug 2011 09:39
Last Modified:10 Aug 2011 09:39

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