Modes of binding of α(1-2) linked manno-oligosaccharides to concanavalin A

Abstract

Three-dimensional structures of the complexes of concanavalin A (ConA) with α (1-2) linked mannobiose, triose and tetraose have been generated with the X-ray crystal structure data on native ConA using the CCEM (contact criteria and energy minimization) method. All the constituting mannose residues of the oligosaccharide can reach the primary binding site of ConA (where methyl-α -d-mannopyranose binds). However, in all the energetically favoured complexes, either the non-reducing end or middle mannose residues of the oligosaccharide occupy the primary binding site. The middle mannose residues have marginally higher preference over the non-reducing end residue. The sugar binding site of ConA is extended and accommodates at least three α (1-2) linked mannose residues. Based on the present calculations two mechanisms have been proposed for the binding of α (1-2) linked mannotriose and tetraose to ConA.