Induction of gamete compatibility and seed formation in Axenic cultures of a diploid self-incompatible species of Petunia

Rangaswamy, N. S. ; Shivanna, K. R. (1967) Induction of gamete compatibility and seed formation in Axenic cultures of a diploid self-incompatible species of Petunia Nature, 216 . pp. 937-939. ISSN 0028-0836

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Official URL: http://www.nature.com/nature/journal/v216/n5118/ab...

Related URL: http://dx.doi.org/10.1038/216937a0

Abstract

In reviews of the control of fertilization in flowering plants, experimental methods of overcoming self-incompatibility have been discussed. For example, induction of auto-tetraploidy by colchicine produced compatibility in a diploid self-incompatible species of Petunia, but self-pollination of the excised whole pistils cultured in vitro did not, as one of us has shown. We report here the elimination of self-incompatibility in the diploid (n = 7) Petunia axillaris (Lam.) B.S.P. by fertilization in vitro, a technique devised in this laboratory and improved by us.

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