Banerjee, R. K. ; Shertzer, Howard G. ; Kanner, Baruch I. ; Racker, Efraim (1977) Purification and reconstitution of the phosphate transporter from bovine heart mitochondria Biochemical and Biophysical Research Communications, 75 (3). pp. 772-778. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...
Related URL: http://dx.doi.org/10.1016/0006-291X(77)91539-X
Abstract
Phosphate transporter of bovine heart mitochondria was purified by solubilization of submitochondrial particles with octylglucoside and fractionation of the extract with ammonium sulfate. After reconstitution into liposomes the purified protein catalyzed phosphate transport which was sensitive to mersalyl and other SH reagents. Transport measured either as Pi/OH or Pi/Pi exchange was proportional to protein concentration and time. The Pi/OH but not the Pi/Pi exchange was stimulated several fold by valinomycin plus nigericin in the presence of K+. The reconstituted system provides a suitable assay during purification of the mitochondrial phosphate transporter.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 5222 |
| Deposited On: | 18 Oct 2010 06:59 |
| Last Modified: | 14 May 2011 09:29 |
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