Singh, D. V. ; Verma, R. K. ; Gupta, M. M. ; Sushil Kumar, (2002) Quantitative determination of oleane derivatives in Terminalia arjuna by high performance thin layer chromatography Phytochemical Analysis, 13 (4). pp. 207-210. ISSN 0958-0344
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/pca.643...
Related URL: http://dx.doi.org/10.1002/pca.643
Abstract
A simple, precise and rapid high performance thin layer chromatographic method has been developed for the simultaneous quantitative determination of five oleane derivatives, namely, arjunic acid, arjunolic acid, arjungenin, arjunetin and arjunglucoside I from stem bark extract of Terminalia arjuna. The isolation and separation of these compounds was carried out on 60F254 layers eluted with chloroform:methanol (90:10), and the analytes were visualised through colour development with vanillin in concentrated sulphuric acid:ethanol. Scanning and quantification of the spots at 640 nm showed good recoveries in the range 96.40-101.7%.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| Keywords: | High Performance Thin Layer Chromatography; Quantitative Determination: Triterpenes; Oleane Derivatives: Terminalia arjuna |
| ID Code: | 52161 |
| Deposited On: | 02 Aug 2011 07:55 |
| Last Modified: | 02 Aug 2011 07:55 |
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