Verma, R. K. ; Bhartariya, K. G. ; Gupta, M. M. ; Sushil Kumar, (1999) Reverse-phase high performance liquid chromatography of asiaticoside in Centella asiatica Phytochemical Analysis, 10 (4). pp. 191-193. ISSN 0958-0344
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1...
Related URL: http://dx.doi.org/10.1002/(SICI)1099-1565(199907/08)10:4<191::AID-PCA456>3.0.CO;2-R
Abstract
A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of asiaticoside, a pharmacologically active constituent from Centella asiatica. Using an octadecyl silane-packed column eluted with water (containing 1% trifluoroacetic acid):methanol (30:70, v/v), asiaticoside was well resolved from other constituents. These optimized analytical conditions allowed the determination of asiaticoside with a recovery of 97%.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| Keywords: | Anti-leprosy; Asiaticoside; Reverse-phase High Performance Liquid Chromatography; Centella asiatica; Umbelliferae |
| ID Code: | 52149 |
| Deposited On: | 02 Aug 2011 07:49 |
| Last Modified: | 02 Aug 2011 07:49 |
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