Isolation of genomic DNA from the antimalarial plant Artemisia annua

Abstract

A suitable protocol is described to obtain high molecular weight, restrictable, and amplifiable genomic DNA from the antimalarial plant Artemisia annua. The method is a CTAB procedure that includes a rapid micro-column chromatography through DE-52 ion- exchange resin. The pure consistency of the template may also offer a reliability advantage for PCR and RFLP based applications. Restriction of the purified DNA with DraI and HindIII resulted in discrete bands that may have arisen due to some repeat sequences equally spaced between the restriction site(s). Perhaps this implies a scope for direct identification of some repeat sequences such as putative species-specific probes.