Recessive monogenic mutation in grain pea (pisum sativum) that causes pyridoxine requirement for growth and seed production

Abstract

A stable pyridoxine-deficient pea mutant was obtained by screening the M2 progeny of azide-treated Pisum sativum cv Pusa Harbhajan. The mutation is visible lethal. The isolation of pyridoxine-deficient mutant demonstrates directly that pea plants synthesize their own pyridoxine and that pyridoxine is an essential growth factor for pea plants. The mutant character is determined by homozygous recessive alleles, designatedpdx-1, at a single locus. Pyridoxine-deficient plants are fertile and indistinguishable from the wild type if supplied exogenously with 2 mg of pyridoxine.