Raval, Aparna ; Puri, Niti ; Saxena, R. K. (1997) Generation of human class I major histocompatibility complex activating factor in serum free medium and its partial characterization Journal of Biosciences, 22 (1). pp. 59-68. ISSN 0250-5991
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Official URL: http://www.ias.ac.in/jarch/jbiosci/22/59-68.pdf
Related URL: http://dx.doi.org/10.1007/BF02703618
Abstract
Human peripheral blood mononuclear cells (PBMCs) activated with Con-A release a soluble factor which augments the expression of class I major histocompatibility complex (MHC) antigens by a variety of tumour cells. Previous attempts to purify this factor called MHC-activating factor (AF) (MHC-AF) made us realize that the presence of large numbers and quantities of irrelevant fetal calf serum proteins in the culture supernatants of the activated human PBMCs, interfered with the purification procedure. It was therefore necessary to standardize the use of a serum free culture medium to generate human MHC-AF. In the present communication we have tried several types of culture media and have identified DCCM-2 as the most suitable culture medium to generate human MHC-AF. MHC-AF generated in DCCM-2 medium appears to be a protein molecule resistant to pH 2 treatment but sensitive to heat treatment (56°C×45 min) and treatment with proteolytic enzymes trypsin and chymotrypsin.
Item Type: | Article |
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Source: | Copyright of this article belongs to Indian Academy of Sciences. |
Keywords: | MHC Antigens; Cytokine; Serum Free Medium; MHC-AF |
ID Code: | 51780 |
Deposited On: | 30 Jul 2011 05:50 |
Last Modified: | 18 May 2016 05:34 |
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