Saxena, Rajiv K. ; Collins, Gary D. ; Chrest, Joseph F. ; Adler, William H. (1996) Bcl-2 transfection protects Hut78 cell line from different types of cytotoxic effector cells Immunology Letters, 52 (2-3). pp. 95-98. ISSN 0165-2478
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0165-2478(96)02590-4
Abstract
The human T lymphoma cell line Hut78 was transfected with Bcl-2 gene or with control G418 Neomycin resistance-conferring plasmid. Bcl-2 transfected and Neomycin selected Hut78 cells expressed about ten-fold greater Bcl-2 protein than the neo-transfected controls. Susceptibility of Bcl-2-transfected Hut78 cells to human NK cells, human mixed lymphocyte reaction-generated cytotoxic effector cells, and mouse cytotoxic spleen cells generated by immunization with Hut78 cells was examined. In all systems tested, Bcl-2-transfected Hut78 target cells were significantly less susceptible to lysis than the neo-transfected control target cells. These results suggest that in Hut78 cells, Bcl-2 gene product may confer partial resistance to different types of cytotoxic effector mechanisms.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Bcl-2 Transfection; Hut78 Cell Line; Cytoxic Effector Cells |
| ID Code: | 51773 |
| Deposited On: | 30 Jul 2011 05:50 |
| Last Modified: | 30 Jul 2011 05:50 |
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