Saxena, Rajiv K. ; Williams, Wanda ; Mcgee, John K. ; Daniels, Mary J. ; Boykin, Elizabeth ; Ian Gilmour, M. (2007) Enhanced in vitro and in vivo toxicity of poly-dispersed acid-functionalized single-wall carbon nanotubes Nanotoxicology, 1 (4). pp. 291-300. ISSN 1743-5390
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Official URL: http://informahealthcare.com/doi/abs/10.1080/17435...
Related URL: http://dx.doi.org/10.1080/17435390701803110
Abstract
Many potential applications in nanotechnology envisage the use of better-dispersed and functionalized preparations of carbon nanotubes. Single-walled carbon nanotubes (SWCNTs) were treated with 1:1 mixtures of concentrated nitric and sulfuric acids for 3 min in a microwave oven under 20 psi pressure followed by extensive dialysis to remove the acids. This treatment resulted in acid functionalized SWCNTs (AF-SWCNTs) that had high negative charge (Zeta potential -40 to -60 mV) and were well dispersed (98% of the particles <150 nm) in aqueous suspensions. In vitro and in vivo toxic effects of SWCNTs and AF-SWCNTs were compared. AF-SWCNTs exerted a strong cytotoxic effect on LA4 mouse lung epithelial cells in culture that could be blocked by prior treatment of the nanotubes with poly L-lysine which neutralized the electric charge and promoted re-agglomeration. AF-SWCNT, but not the unmodified SWCNT preparations, strongly inhibited cell cycling of LA4 cells. Both SWCNTs and AF-SWCNTS were however equally effective in inducing apoptotic responses in LA4 cells as examined using an Annexin V binding assay. Oro-pharyngeal aspiration of AF-SWCNT preparation induced a strong acute inflammatory response in the lungs of CD1 mice, compared to control SWCNTs which caused only a marginal effect. Taken together the results indicate that unlike pristine SWCNTs, acid-functionalized SWCNT preparations exert strong toxic effects in vitro and in vivo and these effects can be reversed by neutralizing their surface charge.
Item Type: | Article |
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Source: | Copyright of this article belongs to Informa plc. |
Keywords: | Nanoparticles; Nanotubes; Particle Toxicology |
ID Code: | 51752 |
Deposited On: | 30 Jul 2011 05:43 |
Last Modified: | 30 Jul 2011 05:43 |
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