Gulati, Ruchi ; Saxena, R. K. ; Gupta, Rani (2000) Fermentation and downstream processing of lipase from Aspergillus terreus Process Biochemistry, 36 (1-2). pp. 149-155. ISSN 0032-9592
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0032-9592(00)00201-6
Abstract
Fermentation behaviour of Aspergillus terreus lipase was studied in a 10 l fermentor. Lipase production was enhanced to 14 200 U l−1 in 54 h in the fermentor as against 7000 U l−1 in 96 h in shake flasks under optimised nutritional conditions. A 2.4-fold increase in specific activity (16.2 U mg−1 protein) was also attained. Inoculum density, dissolved oxygen levels and agitation were the major controlling factors. A two-step cost-effective downstream processing methodology comprising of an aqueous two-phase system (ATPS) of polyethylene glycol (PEG) and phosphate was devised. This procedure resulted in a 12-fold purification of the lipase with 100% yield in less than 1 h.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Lipase; Fermentation; Downstream Processing; Aqueous Two Phase System |
| ID Code: | 51744 |
| Deposited On: | 30 Jul 2011 05:40 |
| Last Modified: | 30 Jul 2011 05:40 |
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