Bist, Pradeep ; Madhusoodanan, Urulangodi Kunhiraman ; Rao, Desirazu N. (2006) A mutation in the mod subunit of EcoP15I restriction enzyme converts the DNA methyltransferase to a site-specific endonuclease Journal of Biological Chemistry, 282 (6). pp. 3520-3530. ISSN 0021-9258
Full text not available from this repository.
Official URL: http://www.jbc.org/content/282/6/3520.abstract?sid...
Related URL: http://dx.doi.org/10.1074/jbc.M603250200
Abstract
A closer inspection of the amino acid sequence of EcoP15I DNA methyltransferase revealed a region of similarity to the PDXn(D/E)XK catalytic site of type II restriction endonucleases, except for methionine in EcoP15I DNA methyltransferase instead of proline. Substitution of methionine at position 357 by proline converts EcoP15I DNA methyltransferase to a site-specific endonuclease. EcoP15I-M357P DNA methyltransferase specifically binds to the recognition sequence 5'-CAGCAG-3' and cleaves DNA asymmetrically EcoP151-M357P·DNA methyltransferase specifically binds to the recognition sequence 5'-CAGCAG-3' and cleaves DNA asymmetrically, 5'-CAGCAG(N)10-3', as indicated by the arrows, in presence of magnesium ions.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to The American Society for Biochemistry and Molecular Biology. |
| ID Code: | 51198 |
| Deposited On: | 28 Jul 2011 07:26 |
| Last Modified: | 28 Jul 2011 07:26 |
Repository Staff Only: item control page
