Lin, Yi-Pin ; Greenwood, Alex ; Nicholson, Linda K. ; Sharma, Yogendra ; McDonough, Sean P. ; Chang, Yung-Fu (2009) Fibronectin binds to and induces conformational change in a disordered region of leptospiral immunoglobulin-like protein B The Journal of Biological Chemistry, 284 (35). pp. 23547-23557. ISSN 0021-9258
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Official URL: http://www.jbc.org/content/284/35/23547.short
Related URL: http://dx.doi.org/10.1074/jbc.M109.031369
Abstract
Leptospira interrogans is a pathogenic spirochete that causes disease in both humans and animals. LigB (Leptospiral immunoglobulin-like protein B) contributes to the binding of Leptospira to extracellular matrix proteins such as fibronectin (Fn), fibrinogen, laminin, and collagen. A high affinity Fn-binding region of LigB has been recently localized to LigBCen2, which contains the partial eleventh and full twelfth immunoglobulin-like repeats (LigBCen2R) and 47 amino acids of the non-repeat region (LigBCen2NR) of LigB. In this study, LigBCen2NR was shown to bind to the N-terminal domain (NTD) of Fn (KD=379 nm) by an enzyme-linked immunosorbent assay and isothermal titration calorimetry. Interestingly, this sequence was not observed to adopt secondary structure by far UV circular dichroism or by differential scanning calorimetry, in agreement with computer-based secondary structure predictions. A low partition coefficient (Kav) measured with gel permeation chromatography, a high hydrodynamic radius (Rh) measured with dynamic light scattering, and the insensitivity of the intrinsic viscosity to guanidine hydrochloride treatment all suggest that LigBCen2NR possesses an extended and disordered structure. Two-dimensional 15N-1H HSQC NMR spectra of intact LigBCen2 in the absence and presence of NTD are consistent with these observations, suggesting the presence of both a β-rich region and an unstructured region in LigBCen2 and that the latter of these selectively interacts with NTD. Upon binding to NTD, LigBCen2NR was observed by CD to adopt a β-strand-rich structure, suggestive of the known β-zipper mode of NTD binding.
Item Type: | Article |
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Source: | Copyright of this article belongs to The American Society for Biochemistry and Molecular Biology. |
ID Code: | 50877 |
Deposited On: | 27 Jul 2011 13:29 |
Last Modified: | 27 Jul 2011 13:29 |
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