Solution structure and calcium-binding properties of M-crystallin, a primordial βγ-crystallin from archaea

Barnwal, Ravi Pratap ; Jobby, M. K. ; Manjula Devi, K. ; Sharma, Yogendra ; Chary, Kandala V. R. (2009) Solution structure and calcium-binding properties of M-crystallin, a primordial βγ-crystallin from archaea Journal of Molecular Biology, 388 (3). pp. 675-689. ISSN 0022-2836

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.jmb.2008.12.058

Abstract

The lens βγ-crystallin superfamily has many diverse but topologically related members belonging to various taxa. Based on structural topology, these proteins are considered to be evolutionarily related to lens crystallins, suggesting their origin from a common ancestor. Proteins with βγ-crystallin domains, although found in some eukaryotes and eubacteria, have not yet been reported in archaea. Sequence searches in the genome of the archaebacterium Methanosarcina acetivorans revealed the presence of a protein annotated as a βγ-crystallin family protein, named M-crystallin. Solution structure of this protein indicates a typical βγ-crystallin fold with a paired Greek-key motif. Among the known structures of βγ-crystallin members, M-crystallin was found to be structurally similar to the vertebrate lens βγ-crystallins. The Ca2+-binding properties of this primordial protein are somewhat more similar to those of vertebrate βγ-crystallins than to those of bacterial homologues. These observations, taken together, suggest that amphibian and vertebrate βγ-crystallin domains are evolutionarily more related to archaeal homologues than to bacterial homologues. Additionally, identification of a βγ-crystallin homologue in archaea allows us to demonstrate the presence of this domain in all the three domains of life.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Lens βγ-crystallins; Calcium Binding; Ancestral Domain; Crystallins; Archaea
ID Code:50862
Deposited On:27 Jul 2011 13:29
Last Modified:27 Jul 2011 13:29

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