Gupta, Jagdish C. ; Raina, Komal ; Talwar, G. P. ; Verma, Rashmi ; Khanna, Navin (2004) Engineering, cloning, and expression of genes encoding the multimeric luteinizing-hormone-releasing hormone linked to T cell determinants in Escherichia coli Protein Expression and Purification, 37 (1). pp. 1-7. ISSN 1046-5928
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.pep.2004.03.018
Abstract
Two synthetic genes were designed and engineered to encode for multimeric luteinizing-hormone-releasing hormone (LHRH) peptides linked to T cell determinants. These genes were cloned into the prokaryotic expression vectors under control of strong inducible promoters, to overexpress the multimeric LHRH peptides as recombinant proteins. Multimeric LHRH-T cell peptides were expressed as insoluble inclusion bodies in Escherichia coli cultures. Cell extracts containing the recombinant proteins showed immunoreactivity on Western blots with monoclonal antibody recognizing the native hormonal peptide. These gene constructs have potential applications in therapy of sex-steroid-hormone-dependent cancers.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Prostate; LHRH Vaccine; T7 Promoter; λPL Promoter |
| ID Code: | 50569 |
| Deposited On: | 26 Jul 2011 13:15 |
| Last Modified: | 26 Jul 2011 13:15 |
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