Bambardekar, Kapil ; Dharmadhikari, Aditya K. ; Sharma, Shobhona ; Dharmadhikari, Jayashree A. ; Mathur , Deepak (2008) Measuring erythrocyte deformability with fluorescence, fluid forces, and optical trapping Journal of Biomedical Optics, 13 (6). 064021_1-064021_7. ISSN 1083-3668
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Official URL: http://link.aip.org/link/?JBOPFO/13/064021/1
Related URL: http://dx.doi.org/10.1117/1.3037342
Abstract
A laser-based method has been developed for experimentally probing single red blood cell (RBC) buckling and determining RBC membrane rigidity. Our method combines a liquid flow cell, fluorescence microscopy, and an optical-trap to facilitate simple measurements of the shear modulus and buckling properties of single RBCs, under physiological conditions. The efficacy of the method is illustrated by studying buckling behavior of normal and Plasmodium-infected RBCs, and the effect of Plasmodium falciparum-conditioned medium on normal, uninfected cells. Our simple method, which quantifies single-RBC deformability, may ease detection of RBC hematological disorders.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to The International Society for Optical Engineering. |
| Keywords: | Red Blood Cells; Malaria; Cell Elasticity; Optical Trap; Erythrocytes |
| ID Code: | 50367 |
| Deposited On: | 23 Jul 2011 09:17 |
| Last Modified: | 17 Apr 2012 06:41 |
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