Probing messenger RNA conformational heterogeneity using single-molecule fluorescence anisotropy

Sinha, Deepak ; Sastry, Srikanth ; Shivashankar, G. V. (2006) Probing messenger RNA conformational heterogeneity using single-molecule fluorescence anisotropy Applied Physics Letters, 88 (10). 103901_1-103901_3. ISSN 0003-6951

Full text not available from this repository.

Official URL: http://apl.aip.org/resource/1/applab/v88/i10/p1039...

Related URL: http://dx.doi.org/10.1063/1.2183358

Abstract

In this letter we describe a method to probe biomolecular conformations and their dynamics at the single molecule level. We show, using fluorescence anisotropy based methods, that the hydrodynamic volume of biomolecules captures the intrinsic heterogeneity within a population. Population distributions of conformations and their dynamics are studied by making anisotropy measurements on one molecule at a time within a confocal volume. The mean anisotropy of mRNA is lowered on addition of salt while the spread remains the same. The intrinsic heterogeneity is revealed when conformational transitions are frozen, resulting in a drastic increase in the spread of the anisotropy. These studies reveal that mRNA samples a broad range of conformations.

Item Type:Article
Source:Copyright of this article belongs to American Institute of Physics.
Keywords:Molecular Biophysics; Molecular Configurations; Fluorescence; Biological Techniques
ID Code:50181
Deposited On:21 Jul 2011 14:28
Last Modified:21 Jul 2011 14:28

Repository Staff Only: item control page